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【医学ppt课件】三氧化二砷抑制人胆囊癌裸鼠移植瘤生长及机制探讨

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医学ppt课件 三氧化二砷抑制人胆囊癌裸鼠移植瘤生长及 人胆囊癌裸鼠移植瘤生长 三氧化二砷抑制 癌裸鼠移植瘤 三氧化二砷及 癌裸鼠移植瘤生长 探讨三氧化二砷 裸鼠移植瘤生长 抑制肿瘤生长
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单击此处编辑母版标题样 式 单击此处编辑母版副标题样式 * * 1 1 * * 三氧化二砷抑制人胆囊癌裸鼠 移植瘤生长及机制探讨 • 复旦大学附属中山医院 • 刘厚宝 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 面临的 困境 • 胆囊癌的发病率逐年上升 • 大多数患者确诊时已属中晚期 • 切除率低 • 放化疗均不敏感 • 5年生存率5% Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 三氧化二砷 • “Proud of China” “Ancient remedy performs new tricks” Science,1996,Aug 2(卷首语) • 在血液系统肿瘤的基础和临床取得突破性 进展 • 在实体瘤的研究领域颇受关注 • 2005年底被批准为治疗原发性肝癌的适应 证 • 对胆囊癌有效??? Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 三氧化二砷和胆囊癌 • 两者之间的研究很少 • 国外 OVID,MEDLINE,ELSEVIER 共2篇,收录于BBRC • 国内 中文科技目录 共15篇 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 临床应用 • 将As2O3用于4例中晚期胆囊癌患者,2个疗 程,1例获得完全缓解(8个月) 钱军,秦叔逵,何泽明,等.三氧化二砷注射液 治疗中晚期原发性肝胆癌的临床研究[J].中 华肿瘤杂志,2001,23(6):487-489 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 目 的 本研究旨在观察As2O3对人胆囊癌裸鼠 移植瘤生长的抑制作用并探讨其作用机制, 期冀为将其应用于胆囊癌临床治疗提供理论 依据。 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 中山医院普外科 细胞株的培养收获细胞动物模型建立 胆囊癌细胞 GBC-SD 复苏,转移,培 养,观察生长情 况 雌性裸小鼠 BALB/c-nu/nu 以0.5ml/只,皮 下注射 ,观察瘤 的生长情况 观察细胞生长至 对数生长期 收获细胞,以NS 配置成1×106浓 度 材料与方法 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 中山医院普外科 材料与方法 10mg/kg 动物数 亚砷酸 亚砷酸组阴性对照组 10只 iv,1次/d×7d 0.2ml/只 生理盐水 给药方式 10只 iv,1次/d×7d Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 中山医院普外科 体积瘤重 Cyc lin d1 Bcl-2 表达 每3天观察 实验组及对 照组的肿瘤 体积 观察至21天 处死裸鼠, 测量瘤重, 并计算肿瘤 生长抑制率 免疫组化法测 定两组肿瘤组 织中Cyclin D1蛋白表达 的情况 免疫组化法 测定两组肿 瘤组织中Bcl- 2蛋白表达的 情况 材料与方法 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 中山医院普外科 材料与方法 公式: V(V(瘤体积瘤体积) ) = = a×b2 /2 (aa×b2 /2 (a为长径,为长径,b b为短径为短径) ) 结果在结果在SPSS11.5SPSS11.5软件下进行软件下进行, ,两组均数之间进行两组均数之间进行t t检验,检验,P P 0.050.05表示差异有显著性。表示差异有显著性。 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 两组肿瘤体积的比较 结 果 中山医院普外科 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 结 果 组别 阴性对照组 亚砷酸组 动物数瘤重(g)抑瘤率(%) 10个 10个 0.992±0.401 0.526±0.269 46.98 两组肿瘤瘤重的比较 中山医院普外科 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 免疫组化对Cyclin D1表达的检测 As2O3组Cyclin D1的蛋白表达量较对照组明显下降 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 免疫组化对Bcl-2表达的检测 As2O3组Bcl-2的蛋白表达量较对照组明显下降 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. Western Blot检测Bcl-2、Cyclin D1 蛋白的表达情况 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 讨 论 本研究使用亚砷酸注射液10mg/kg实验,最终发现亚砷 酸组的瘤体积和瘤重均较阴性对照组明显减小。其肿瘤细胞 生长抑制率为46.98%。 说明10mg/kg剂量的亚砷酸能明显抑制人胆囊癌裸鼠移 植瘤的生长,亚砷酸对其起到抗癌作用。 中山医院普外科 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 中山医院普外科 细细胞凋亡 细细胞毒作用 端粒酶 其他 肿肿瘤血管 4. 抑制内皮细胞增生 ,抗肿瘤血管形成, 抑制VEGF的表达 5. 影响肿瘤抑癌基因 ,癌转移基因的表达 ,影响肿瘤细胞的细 胞周期等 1. 上调细胞凋亡 促进基因如Bax, Fas等的表达,下 调抑制基因如Bcl- 2等的表达 2. 选择性的抑制 肿瘤细胞增殖, 并使其破碎坏死 3. 抑制端粒酶活 性亚亚亚亚砷酸砷酸((AsAs 2 2 OO 3 3 ))抗癌抗癌 作用的可能机制作用的可能机制 As2O3 讨 论 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. Cyclin D1 • 细胞周期进程的启动子 • Cyclin D1 上升,更多细胞从静止期进入增殖期,肿 瘤细胞增殖加快 • Cyclin D1 下降,更多细胞从增殖期进入静止期,肿 瘤细胞增殖减缓 文献报道:降低乳腺癌细胞 、肝癌细胞的 Cyclin D1能使癌细胞生长、增殖及侵袭力下降 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. Bcl-2与胆囊癌 • 40 例原发性胆囊癌中28 例Bcl-2反应阳性 • Bcl-2表达与在胆囊癌的分期密切相关 • 胆囊癌组织中细胞凋亡与Bcl-2表达密切相关 • Bcl-2表达与胆囊癌发生发展密切相关 原发性胆囊癌组织中细胞凋亡与相关基因bcl-2和bax 的表达[J].陕西医学杂志,2005,34(2)231~232 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 结 论 AsAs 2 2 OO 3 3 (10mg/kg)(10mg/kg)可抑制人胆囊癌可抑制人胆囊癌 GBCGBC细胞裸鼠移植瘤的生长,主要通过细胞裸鼠移植瘤的生长,主要通过 下调肿瘤组织下调肿瘤组织Cyclin D1Cyclin D1和和Bcl-2Bcl-2的表达的表达 从而改变其异常的细胞周期和细胞凋亡从而改变其异常的细胞周期和细胞凋亡 来实现。这为将来实现。这为将As2O3As2O3应用于胆囊癌的应用于胆囊癌的 综合治疗提供了理论依据。综合治疗提供了理论依据。 中山医院普外科 Through this publicity campaign, further improve the sense of business integrity of tourism enterprises to help tourism consumers and operators to safeguard the legitimate rights and interests, to ensure that our City's tourism sustainable, healthy and coordinated Development. 中山医院普外科 严谨、求实、团结、奉献
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